Bicinchoninic acid

Bicinchoninic acid

IUPAC name 2-(4-carboxyquinolin-2-yl)quinoline-4-carboxylic acid
Other names Bicinchoninic acid 4,4'-Dicarboxy-2,2'-Biquinoline
Identifiers
CAS number	1245-13-2
PubChem	71068
ChemSpider	64223^Y
ChEMBL	CHEMBL431482^Y
Jmol-3D images	Image 1
SMILES O=C(O)c1cc(nc2c1cccc2)c3nc4ccccc4c(c3)C(=O)O
InChI InChI=1S/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26)^Y Key: AFYNADDZULBEJA-UHFFFAOYSA-N^Y InChI=1/C20H12N2O4/c23-19(24)13-9-17(21-15-7-3-1-5-11(13)15)18-10-14(20(25)26)12-6-2-4-8-16(12)22-18/h1-10H,(H,23,24)(H,25,26) Key: AFYNADDZULBEJA-UHFFFAOYAF
Properties
Molecular formula	(HO₂CC₉H₅N)₂
Molar mass	344.33 g/mol
Appearance	Cream powder. Characteristic odor.
Density	Solid in pure form. Density not available.
Melting point	365 to 367 °C
Boiling point	Not available.
Solubility in water	Partially soluble in cold water, hot water.
Hazards
MSDS	External MSDS
Main hazards	Will irritate eyes and mucous membranes. The chemical, physical and toxicological properties have not been fully investigated. Presume moderate toxicity internally. LD/TD no data. OSHA PEL/ACGIH TLV not established. No evidence of carcinogenicity.
NFPA 704	0 1 0
Flash point	N/A
Y (verify) (what is: Y/N?) Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Bicinchoninic acid is a weak acid composed of two carboxylated quinoline rings.

Bicinchoninic acid is most commonly employed by biochemists in the bicinchoninic acid assay, which is used to determine the total level of protein in a solution. In this assay, two molecules of bicinchoninic acid chelate a single Cu¹⁺ ion, forming a purple water-soluble complex that strongly absorbs light at 562 nm.^[1]

References

^ Pierce BCA Protein Assay Kit